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A Fast and Accessible Methodology for Micro-Patterning Cells on Standard Culture Substrates Using Parafilm™ Inserts

Micropatterning techniques to control the spatial organization of cells at the sub-mm scale are useful for tissue engineering [1], biosensor technology development [2], and for asking fundamental questions about the dependence of cell behaviour on local tissue organization [3], [4]. Micropatterning techniques provide direct control over several spatial parameters including colony or cell sheet size, distance between colonies, and with some methods, homotypic or heterotypic cell–cell contact [5], [6].

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A Fast and Accessible Methodology for Micro-Patterning Cells on Standard Culture Substrates Using Parafilm™ Inserts

Micropatterning techniques to control the spatial organization of cells at the sub-mm scale are useful for tissue engineering [1], biosensor technology development [2], and for asking fundamental questions about the dependence of cell behaviour on local tissue organization [3], [4]. Micropatterning techniques provide direct control over several spatial parameters including colony or cell sheet size, distance between colonies, and with some methods, homotypic or heterotypic cell–cell contact [5], [6].

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A Fast and Accessible Methodology for Micro-Patterning Cells on Standard Culture Substrates Using Parafilm™ Inserts

Micropatterning techniques to control the spatial organization of cells at the sub-mm scale are useful for tissue engineering [1], biosensor technology development [2], and for asking fundamental questions about the dependence of cell behaviour on local tissue organization [3], [4]. Micropatterning techniques provide direct control over several spatial parameters including colony or cell sheet size, distance between colonies, and with some methods, homotypic or heterotypic cell–cell contact [5], [6].

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