The evolution of tissue form in development, wound healing, and regeneration is a dynamic process that involves the integration of local cues on cell fate and function. These cues include interactions with soluble factors (growth factors, morphogens, dissolved gases) and insoluble factors (extracellular matrix, neighboring cells) in a three-dimensional context. A fundamental understanding of how tissue structure evolves is critical to the rational development of engineered tissues for therapeutic applications.

Vibrio mimicus occasionally causes sporadic diarrhea and extraintestinal infections. Although it was previously recognized as a biotype of V. cholerae, it has now been reclassified as an independent species because of differences in a number of biochemical characteristics; e.g., V. mimicus is negative for sucrose fermentation, Voges-Proskauer, lipase (corn oil) activity, and Jordan tartrate reactions.

Micropatterning techniques to control the spatial organization of cells at the sub-mm scale are useful for tissue engineering [1], biosensor technology development [2], and for asking fundamental questions about the dependence of cell behaviour on local tissue organization [3], [4]. Micropatterning techniques provide direct control over several spatial parameters including colony or cell sheet size, distance between colonies, and with some methods, homotypic or heterotypic cell–cell contact [5], [6].