Biomedical Engineering

In-Silico Patterning of Vascular Mesenchymal Cells in Three Dimensions

The evolution of tissue form in development, wound healing, and regeneration is a dynamic process that involves the integration of local cues on cell fate and function. These cues include interactions with soluble factors (growth factors, morphogens, dissolved gases) and insoluble factors (extracellular matrix, neighboring cells) in a three-dimensional context. A fundamental understanding of how tissue structure evolves is critical to the rational development of engineered tissues for therapeutic applications.

Genome Sequencing Reveals Unique Mutations in Characteristic Metabolic Pathways and the Transfer of Virulence Genes between V. mimicus and V. cholerae

Vibrio mimicus occasionally causes sporadic diarrhea and extraintestinal infections. Although it was previously recognized as a biotype of V. cholerae, it has now been reclassified as an independent species because of differences in a number of biochemical characteristics; e.g., V. mimicus is negative for sucrose fermentation, Voges-Proskauer, lipase (corn oil) activity, and Jordan tartrate reactions.

Efficient Immunoglobulin Gene Disruption and Targeted Replacement in Rabbit Using Zinc Finger Nucleases

Rabbits are important laboratory animals, widely used in many areas of biomedical research, including the production of antibodies and recombinant proteins. Rabbit models have contributed to the understanding of human diseases and the development of therapeutic compounds, devices and techniques.

Ultrasound for Autonomous Heart Attacks

Every year, half of the 610,000 heart attack deaths in the United States are due to a lack of prompt medical attention. Currently, most technologies fail to fix the problem, as they simply take too long to be able to detect a heart attack in time.

CRISPR Inhibition of Prophage Acquisition in Streptococcus Pyogenes

During evolution, bacteria acquired new traits primarily by horizontal gene transfer (HGT) as a key driving force for expressing novel pathogenic properties, new colonization niches as well as metabolic adaptations [1], [2], [3], [4], [5]. Conjugation, transduction and transformation are the major mechanisms for HGT. The contributions and the impact of each mechanism are variable among species [6].

A Coarse-Grained Approach to Protein Design: Learning from Design to Understand Folding

Computational studies have given a great contribution in building our current understanding of the complex behavior of protein molecules; nevertheless, a complete characterization of their free energy landscape still represents a major challenge. Here, we introduce a new coarse-grained approach that allows for an extensive sampling of the conformational space of a large number of sequences.

A Fast and Accessible Methodology for Micro-Patterning Cells on Standard Culture Substrates Using Parafilm™ Inserts

Micropatterning techniques to control the spatial organization of cells at the sub-mm scale are useful for tissue engineering [1], biosensor technology development [2], and for asking fundamental questions about the dependence of cell behaviour on local tissue organization [3], [4]. Micropatterning techniques provide direct control over several spatial parameters including colony or cell sheet size, distance between colonies, and with some methods, homotypic or heterotypic cell–cell contact [5], [6].

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