Modeling the effects of cryopreservation on kidney bean plants to learn which environment will best suit the plant for efficient growth and sustainability.
Contents
Introduction
Materials/Methods
Results
Conclusion
Bibliography
Introduction
What is Cryopreservation? Cryopreservation is the preservation of cells by subjection to extremely low temperatures. Where is cryopreservation used? Cryopreservation is used to store seeds that are vulnerable to extinction. Cryopreservation is used to store donated blood in blood banks.
Materials/Methods
Results
Plant Height in Centimeters |
|||
Water 1 |
Day 8 |
Day 11 |
Day 14 |
Trial 1 |
0 |
9.2 |
36.8 |
Trial 2 |
11.7 |
13.3 |
21.2 |
Trial 3 |
0.25 |
22.3 |
42.3 |
Trial 4 |
0.75 |
9 |
25 |
Trial 5 |
24.6 |
33.5 |
41 |
Avg. |
7.46 |
17.46 |
33.26 |
Plant Height in Centimeters |
|||
Dry Ice 1 |
Day 8 |
Day 11 |
Day 14 |
Trial 1 |
0 |
26.7 |
48.2 |
Trial 2 |
7.3 |
32.7 |
42 |
Trial 3 |
0 |
31.2 |
45.2 |
Trial 4 |
0 |
13.6 |
34 |
Trial 5 |
0 |
16.9 |
37 |
Avg. |
1.46 |
24.22 |
41.28 |
Plant Height in Centimeters |
|||
Water 2 |
Day 8 |
Day 11 |
Day 14 |
Trial 1 |
0.8 |
21.7 |
37 |
Trial 2 |
0 |
22.3 |
42.3 |
Trial 3 |
0 |
1 |
36.1 |
Trial 4 |
0 |
13.9 |
27.2 |
Trial 5 |
1 |
29.6 |
47 |
Avg. |
0.36 |
17.7 |
37.92 |
The line graph shows that the seeds which had been preserved in the dry ice for one hour grew the tallest at an average of 41.28 centimeters. The seeds that had been preserved with dry ice for two hours grew the shortest at an average height of 8.8 centimeters. The seeds that had been preserved in the dry ice for three hours grew the second tallest at an average height of 38.84 centimeters. The seeds that grew the third tallest were the ones preserved with two hours of water at an average height of 37.92 centimeters. The seeds that grew the fourth tallest were the ones preserved with dry ice for four hours at an average height of 35.1 centimeters. The seeds that grew fifth tallest were the seeds preserved with water for one hour, which grew to an average of 33.26 centimeters. The seeds that grew the sixth tallest were the ones preserved with dry ice for five hours which grew an average of 31.04 centimeters. The seeds that grew seventh tallest were the ones preserved with water for three hours, which grew to an average of 38.84 centimeters. The seeds that grew eighth tallest were the ones preserved with water for four hours that grew to an average height of 22.56 centimeters. The seeds that grew the ninth tallest were the ones grown with water for five hours which grew to an average height of 22.22 centimeters.
Conclusion
The researcher asked the question of whether dry ice or water preserved plants better. The researcher believed that the seeds preserved with the dry ice for one hour would grow the tallest. The researcher’s hypothesis was supported. The background information supported the conclusion because the seeds stored using water were structurally damaged. Also, the seeds that had been preserved longer had a larger chance of their DNA being affected.
Some sources of experimental error were that the seeds should have been selectively bred; meaning all seeds for the experiment should have come from the same parent plant. Another source of experimental error is the fact that the water was not poured on the exact same location for each plant, meaning only some of the water could have fell on the plant. From my project I learned the processes a scientist follows to reach conclusions. I learned how to correctly care for plants and how to be accurate when measuring variables. A benefit to society is the project shows how to properly store plants to protect them from disasters and potentially extinction. A question the researcher had is whether different seeds would react differently to the experiment.
Bibliography
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Modeling the effects of cryopreservation on kidney bean plants to learn which environment will best suit the plant for efficient growth and sustainability.