Quantification of Atherosclerosis

Atherosclerosis and subsequent cardiovascular disease causes nearly one-third of all deaths in the world. Unfortunately, atherosclerosis commonly remains asymptomatic for decades, and is properly diagnosed only after a severe, life-threatening cardiac event. Thus, a portable, low‐cost tattoo‐based biosensor for the non‐invasive self‐diagnosis and quantification of atherosclerosis was developed.

Summary of Research

Atherosclerosis and subsequent cardiovascular disease causes nearly one-third of all deaths in the world. Unfortunately, atherosclerosis commonly remains asymptomatic for decades, and is properly diagnosed only after a severe, life-threatening cardiac event. A simple, portable, and inexpensive method for early detection of atherosclerosis is highly desirable. This research details the fabrication of a cost-effective and portable tattoo-based system for the detection and quantification of atherosclerosis progression. The system is contained within a screen-printed biosensor which utilizes human immune response in order to quantify macrophage concentration in the bloodstream via the transdermal iontophoretic administration of sperminesilver nanoparticles (spAg-NPs).

In clinical usage, the patch is placed on the skin directly above the carotid bifurcation, where plaque quantification/diagnostic accuracy is especially high. The patch utilizes an iontophoretic circuit to introduce spAg-NPs into the interstitial fluid matrix. The patient then waits for macrophages to enter into the matrix and engulf a portion of the spAg-NPs. Remaining spAg-NPs are then extracted via a reversed current, which then react with amine oxidase to produce H2O2, which in turn electrochemically reduces Prussian-Blue ‘artificial peroxidase’. Current produced is measured to determine concentration of spAg-NPs, and thus the concentration of macrophages in the bloodstream, which correlates directly with progression of the atherosclerotic plaque. The biosensor demonstrated a limit of detection of 3.26×10-5 M spAg-NPs, with a sensitivity of 5.07 μA/μM-cm2, allowing for the precise, costeffective detection of as little as 0.059% arterial cross-sectional plaque buildup within the carotid bifurcation.

Leave a Reply

Quantification of Atherosclerosis

Atherosclerosis and subsequent cardiovascular disease causes nearly one-third of all deaths in the world. Unfortunately, atherosclerosis commonly remains asymptomatic for decades, and is properly diagnosed only after a severe, life-threatening cardiac event. Thus, a portable, low‐cost tattoo‐based biosensor for the non‐invasive self‐diagnosis and quantification of atherosclerosis was developed.

Scroll to top

2018 ScienceBuzz Symposium

Click Here to go to the Conference Website

We are excited to announce the first annual ScienceBuzz Symposium, dedicated to middle and high school students. The conference and registration is completely free! Currently, the symposium date is June 9, 2018.

Pre-register for the symposium by visiting the conference website or clicking here. There are a limited number of seats and we expect to fill up quickly, so register ASAP. The event will include several internationally-renowned speakers, workshop sessions, company exhibitions, scholarships, and research presentations. 

 

The 2018 ScienceBuzz Symposium is the first, completely free conference exclusively for middle and high school students. Our theme for this year is “One Day of Powerful Talks.”

It will be hosted in Lehigh Valley, Pennsylvania, but will include students from around the state and nearby regions. The event will have national guest keynote speakers (including a Nobel Prize Laureate, the head of a leading scientific institution, college admissions officers, university professors, leaders in gifted education, and top biopharma executives). It will also include workshop sessions, student poster presentations, five research scholarship awards, organizational and company exhibitions, and unique networking opportunities.

To learn more about the symposium, agenda, and logistical details, visit our website at www.sciencebuzz.wixsite.com/symposium

Support us by contributing to our Kickstarter campaign: